Beacon designer software10/29/2022 ![]() enterica is the most clinically significant, causing 99% of Salmonella infections. enterica is further divided into six subspecies, of which S. The CDC distinguishes two Salmonella species (or subgenera): S. The detection of Salmonella therefore remains a highly important issue in microbiological analysis for food safety and standards.īecause the nomenclature for the Salmonella genus is at times confusing, this publication will follow the current literature. The bacterium can be isolated from raw meat and poultry products as well as from milk and milk-based products. Salmonella outbreaks are linked to unhygienic food preparation, cooking, reheating and storage practices. It is the pathogenic agent of salmonellosis, a major cause of enteric illness and typhoid fever, leading to many hospitalisations and a few rare deaths if no antibiotics are administered. Salmonella is a gram-negative, facultative anaerobic, flagellated bacterium. The assay can amplify and analyse a large number of samples in approximately 8 hours, compared to the 4 to 5 days conventional identification takes, and is thus considered a very promising method for detecting the two major serotypes of Salmonella quickly and accurately from clinical and environmental samples. The detection limit was down to 10 copies of DNA target per 25 μl reaction. Therefore, the entire experiment had specificity and sensitivity of 100%. The assay was 100% sensitive and specific, correctly identifying all 44 Salmonella strains, all 21 samples of S. The results were compared to those of the Kauffmann-White antigenic classification scheme. ![]() Three sets of primers were used for the amplification of the target sequences. An internal amplification positive control was included in the experiment to ensure the optimal functioning of the PCR and detect possible PCR inhibition. Salmonella Enteritidis and Salmonella Typhimurium, respectively, the two most clinically relevant serotypes. Target sequences were regions of the invA, prot6E and fliC genes specific for Salmonella spp. ![]() Molecular beacons were incorporated into the assay as probes for target DNA. A fast and simple two-step multiplex real-time PCR assay has been developed to replace the traditional, laborious Salmonella serotyping procedure. ![]()
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